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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC22E12.11c
Gene ID 38/E02
Gene synonyms/obsolete
Gene product involved in transcriptional regulation; zinc finger protein; zf-PHD finger; SET domain; histone lysine methyltransferase
Entry clone Cloned
ORF length (unspliced) 2580
ORF length (spliced)
Entry clone length 2580
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC22E12.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTGGAAAATACGATGCGT
Rev primer name SPAC22E12.11.Rv
Rev primer SEQ AGAAAGCTGGGTATCGCGTAAATGAAGGGTTA
Amino acid length 859
Molecular weight 94.8
Isoelectric point (calc.) 9.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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