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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC19G12.02c
Gene ID 38/E10
Gene synonyms/obsolete pms1
Gene product involved in DNA repair; involved in mismatch repair; MMR pathway; mismatch-binding; mutL homolog
Entry clone Cloned
ORF length (unspliced) 2599
ORF length (spliced) 2385
Entry clone length 2599
No. of intron 3
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC19G12.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCAACGGTTAAACCTAT
Rev primer name SPAC19G12.02.Rv
Rev primer SEQ AGAAAGCTGGGTATATATCTTTTAATCGAAGG
Amino acid length 794
Molecular weight 88
Isoelectric point (calc.) 9.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
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