Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC4F10.07c
Gene ID 38/E11
Gene synonyms/obsolete
Gene product involved in autophagy
Entry clone Cloned#
ORF length (unspliced) 2599
ORF length (spliced) 2277
Entry clone length 2599
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC4F10.07.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCGAGACTTAATACGCA
Rev primer name SPAC4F10.07.Rv
Rev primer SEQ AGAAAGCTGGGTAATCATCGGATTTGGGAGAA
Amino acid length 758
Molecular weight 83.7
Isoelectric point (calc.) 8.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization changed to: nucleus; nuclear dots
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images
LMB 4 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.