Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPAC9.05
Gene ID 38/F02
Gene synonyms/obsolete
Gene product DEAD/DEAH box helicase; ATP-dependent; RNA helicase; similar to Sp SPAC20H4.04 (paralog)
Entry clone Cloned in 2006 trial
ORF length (unspliced) 2606
ORF length (spliced) 2520
Entry clone length 2606
No. of intron 2
Sequence status Partially sequenced
Sequence results 100% match in both ends
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC9.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCGATGATTCTTTTAG
Rev primer name SPAC9.05.Rv
Rev primer SEQ AGAAAGCTGGGTAAATCAGCATTCCTTTCATA
Amino acid length 839
Molecular weight 97.2
Isoelectric point (calc.) 9.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LATFAYPLNL
Localization (YFP) nucleolus>nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.