Gene name |
SPAC13G6.12c |
Gene ID |
38/F11 |
Gene synonyms/obsolete |
chs1;
SPAC24B11.01c |
Gene product |
chitin synthase 1;
chitin synthase activity; essential; involved in sporulation
(required) |
Entry clone |
Cloned |
ORF length (unspliced) |
2626 |
ORF length (spliced) |
2580 |
Entry clone length |
2626 |
No. of intron |
1 |
Sequence status |
Finished |
Sequence results |
536A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC13G6.12.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGAAGGTGGTTCAAAAA |
Rev primer name |
SPAC13G6.12.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTCTCCCGTACAAAGTCTA |
Amino acid length |
859 |
Molecular weight |
97.9 |
Isoelectric point (calc.) |
8.5 |
Signal SEQ |
|
No. of transmembrane domain |
7 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LYPTITNLSL/LTWILSNLFL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |