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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC3A11.06
Gene ID 38/F12
Gene synonyms/obsolete mvp1
Gene product phosphoinositide binding; PX domain; involved in intracellular protein transport
Entry clone Cloned; Mixture
ORF length (unspliced) 2627
ORF length (spliced) 1955
Entry clone length 2627
No. of intron 9
Sequence status Finished
Sequence results Mixture
Comments Mixture of 2 clones, one of which is frameshifted from 205.
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC3A11.06.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGCGATAATGTATTTTT
Rev primer name SPAC3A11.06.Rv
Rev primer SEQ AGAAAGCTGGGTAAGTTTCAAGCCGAGAAAAA
Amino acid length 664
Molecular weight 76.8
Isoelectric point (calc.) 7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) Golgi; vacuole membrane
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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