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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1921.05
Gene ID 38/G07
Gene synonyms/obsolete ape1
Gene product aminopeptidase I; peptidase family M1
Entry clone Cloned
ORF length (unspliced) 2649
ORF length (spliced)
Entry clone length 2649
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1921.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAAGTCACCATTCCTAA
Rev primer name SPBC1921.05.Rv
Rev primer SEQ AGAAAGCTGGGTACATATAACGATTCTCCTTA
Amino acid length 882
Molecular weight 99.4
Isoelectric point (calc.) 5.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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