Gene name |
SPAC8C9.14 |
Gene ID |
38/H04 |
Gene synonyms/obsolete |
prr1 |
Gene product |
heat shock
transcription factor; involved in oxidative stress response
(implicated); essential; involved in expression of oxidative
stress genes (ctt1 and trr1) (required); involved in
expression of sexual development genes (required); response
regulator receiver domain |
Entry clone |
Cloned |
ORF length (unspliced) |
2669 |
ORF length (spliced) |
1620 |
Entry clone length |
2669 |
No. of intron |
4 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC8C9.14.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCGTCTTCGAACGGATC |
Rev primer name |
SPAC8C9.14.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATGTGGATGCTGTAAGTCT |
Amino acid length |
539 |
Molecular weight |
60 |
Isoelectric point (calc.) |
6.2 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LVKPFTKLTL |
Localization (YFP) |
nucleus; nuclear
dots |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |