| Gene name |
SPAC823.12 |
| Gene ID |
39/A09 |
| Gene synonyms/obsolete |
|
| Gene product |
involved in
intracellular protein transport; 7 fold repeat domain; zinc
finger protein; zf-C3HC4 type (RING finger); ubiqitin ligase
(E3) |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2721 |
| ORF length (spliced) |
|
| Entry clone length |
2721 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1507G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC823.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCAACGTTCATTAAAAA |
| Rev primer name |
SPAC823.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATAACCATCGACAACGAATA |
| Amino acid length |
906 |
| Molecular weight |
102.5 |
| Isoelectric point (calc.) |
5.6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCQKMMYNLAI |
| Localization (YFP) |
cytosol |
| Comments for localization |
cytoplasmic dots by
over expression? |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |