| Gene name |
SPBC3D6.12 |
| Gene ID |
39/B03 |
| Gene synonyms/obsolete |
|
| Gene product |
Trp Asp repeat protein
WD repeat protein; Sc DIP2 is dom34 interacting protein;
ribonucleoprotein (RNP) complex; processome component;
involved in rRNA processing |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2769 |
| ORF length (spliced) |
|
| Entry clone length |
2769 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
666T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC3D6.12.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTCAAAAGCTATACGCG |
| Rev primer name |
SPBC3D6.12.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAACAATGTTGGAAAAGGCT |
| Amino acid length |
922 |
| Molecular weight |
103.4 |
| Isoelectric point (calc.) |
5.7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
310 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LDSLKFFLNL/LDQEIQYRLDL/LVLPFEHVLSL |
| Localization (YFP) |
nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
Leica |