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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC550.03c
Gene ID 39/B10
Gene synonyms/obsolete
Gene product putative DEAD/DEAH box helicase DEAD/DEAH box helicase; Sc SKI2 is part of a system to protect cells from double-stranded RNA (dsRNA) viruses
Entry clone Cloned
ORF length (unspliced) 3642
ORF length (spliced)
Entry clone length 3642
No. of intron 0
Sequence status Finished
Sequence results 534A:G / 543C:T / 597A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC550.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTTCTAAACTTGTAGA
Rev primer name SPCC550.03.Rv
Rev primer SEQ AGAAAGCTGGGTACATATACAGTGAAGGGCAG
Amino acid length 1213
Molecular weight 138
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLRVETLRI
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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