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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC146.13c
Gene ID 39/C03
Gene synonyms/obsolete myo1
Gene product putative myosin I protein myosin I; actin cortical patch component; involved in F-actin assembly; involved in cytokinesis; involved in actin cortical patch distribution; type I myosin; involved in cell cycle dependent actin filament organization; src (SH3) homology domain; IQ domains
Entry clone Cloned
ORF length (unspliced) 3698
ORF length (spliced) 3654
Entry clone length 3698
No. of intron 1
Sequence status Finished
Sequence results 408T:C / 1557G:A / 3253C:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC146.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGTAAGTAAATATGCATG
Rev primer name SPBC146.13.Rv
Rev primer SEQ AGAAAGCTGGGTACCAATCTTCTTCTTCATCA
Amino acid length 1217
Molecular weight 135.7
Isoelectric point (calc.) 9.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LQQIFIELTL/LHQIKYLGL
Localization (YFP) periphery; cytoplasmic dots
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica, DeltaVision

Image information
YFP 3 images) See all images

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