| Gene name |
SPAC6G10.05c |
| Gene ID |
39/C05 |
| Gene synonyms/obsolete |
|
| Gene product |
hypothetical protein
TRAPP; involved in intracellular protein transport |
| Entry clone |
Cloned |
| ORF length (unspliced) |
3739 |
| ORF length (spliced) |
3633 |
| Entry clone length |
3739 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
1751T:C / 1826T:C /
3284A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC6G10.05.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAATTTGATTTTTTTTC |
| Rev primer name |
SPAC6G10.05.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACTTCGCATCAACAATTAGT |
| Amino acid length |
1210 |
| Molecular weight |
137.7 |
| Isoelectric point (calc.) |
7.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LELFTVCLVI/LSDEMANNLSI |
| Localization (YFP) |
SPB?; periphery at
site of septum formation |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |