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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC11E3.01c
Gene ID 39/D03
Gene synonyms/obsolete SPAC2H10.03c
Gene product putative Snf2 family helicase SNF2 family; helicase C-terminal domain; similar to human Snf2p homolog; involved in transcriptional regulation; chromodomain-helicase-binding protein
Entry clone Cloned
ORF length (unspliced) 3867
ORF length (spliced)
Entry clone length 3867
No. of intron 0
Sequence status Finished
Sequence results 3121A:G / 3161A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC11E3.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTTACGAGGAGTCAGA
Rev primer name SPAC11E3.01.Rv
Rev primer SEQ AGAAAGCTGGGTACCATTCATCACTAGTTCCT
Amino acid length 1288
Molecular weight 149.4
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 397/183
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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