Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPCC1672.11c
Gene ID 39/D07
Gene synonyms/obsolete
Gene product membrane ATPase; P-type ATPase; P5 type; unknown specificity
Entry clone Cloned
ORF length (unspliced) 3948
ORF length (spliced)
Entry clone length 3948
No. of intron 0
Sequence status Finished
Sequence results 649G:A / 814C:T / 2912A:G / 2919T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCC1672.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCATCTCCGAAGATGAT
Rev primer name SPCC1672.11.Rv
Rev primer SEQ AGAAAGCTGGGTAAACTTGTTCATTTTGCAAG
Amino acid length 1315
Molecular weight 148.7
Isoelectric point (calc.) 6.5
Signal SEQ
No. of transmembrane domain 10
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LYQAICLLTL/LKSVSQLLI/LGDFQFLFI
Localization (YFP) ER
Comments for localization aggregates by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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