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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC1834.08
Gene ID 39/E07
Gene synonyms/obsolete mak1
Gene product putative sensory transduction histidine kinase histidine kinase; involved in signal transduction; His-to-Asp phosphorelay; non-essential; functions upstream of Spy1p; function overlapping with Mak2p and Mak3p; implicated in mitotic cell cycle control; implicated in oxidative stress response; required for the regulation of sexual development; PAC domain protein; similar to Sp mak2 and mak3; response regulator receiver domain
Entry clone Cloned
ORF length (unspliced) 5020
ORF length (spliced) 4920
Entry clone length 5020
No. of intron 1
Sequence status Finished
Sequence results 1969T:C / 3058A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC1834.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGGCCACCTGACGATCA
Rev primer name SPAC1834.08.Rv
Rev primer SEQ AGAAAGCTGGGTATTTGTGTAACTTTGATTGG
Amino acid length 1639
Molecular weight 184.5
Isoelectric point (calc.) 7.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 641
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LLTVINDILDL
Localization (YFP) cytoplasmic dots at cell tip and site of septum formation; cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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