Gene name |
SPBC1D7.02 |
Gene ID |
39/E12 |
Gene synonyms/obsolete |
scr1;
SPBC1D7.02c |
Gene product |
glucose mediated
repressor of Inv1 transcriptional regulator; zinc finger
protein; zf-C2H2 type; glucose mediated repressor of inv1 and
fbp1 |
Entry clone |
Cloned |
ORF length (unspliced) |
1698 |
ORF length (spliced) |
|
Entry clone length |
1698 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC1D7.02.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCGAAGCCACCACTGC |
Rev primer name |
SPBC1D7.02.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGGCTTGGTCATAGGAGTT |
Amino acid length |
565 |
Molecular weight |
59.7 |
Isoelectric point (calc.) |
10.6 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LPSIRSLSL |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
nucleus>cytosol |
Microscope used for
observation |
Leica |