Chemical Genetics Laboratory
PREVIOUS  NEXT
Schizosaccharomyces pombe Postgenome Database

Gene name SPBC25H2.02
Gene ID 39/F07
Gene synonyms/obsolete ths1
Gene product threonyl-tRNA synthetase; cytoplasmic threonyl-tRNA synthetase; involved in threonyl-tRNA aminoacylation; threonine-tRNA ligase activity
Entry clone Cloned
ORF length (unspliced) 2112
ORF length (spliced)
Entry clone length 2112
No. of intron 0
Sequence status Finished
Sequence results 107A:G / 481A:G / 840A:G / 876T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC25H2.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGCGCAGCTGCTGTTAA
Rev primer name SPBC25H2.02.Rv
Rev primer SEQ AGAAAGCTGGGTACAGGTCGTTAATCAGACTT
Amino acid length 703
Molecular weight 80.1
Isoelectric point (calc.) 7.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol
Comments for localization
Effect of LMB on protein localization changed to: cytosol=nucleus
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images
LMB 2 images) See all images

For plasmid request Click!
Copyright (c) RIKEN (The Institute of Physical and Chemical Research), Japan. All rights reserved.