Gene name |
SPBC15D4.03 |
Gene ID |
39/F12 |
Gene synonyms/obsolete |
slm9 |
Gene product |
WD repeat protein;
involved in mitotic control WD repeat protein; involved in
mitotic control; pleiotrophic signal transducer for cell
growth; similar to Sp SPBC15D4.03 |
Entry clone |
Cloned (also cloned in
2004 trial) |
ORF length (unspliced) |
2424 |
ORF length (spliced) |
|
Entry clone length |
2424 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC15D4.03.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCACATTTTTGTGCCTAA |
Rev primer name |
SPBC15D4.03.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATAAAAGTGCAGATCGTCGT |
Amino acid length |
807 |
Molecular weight |
90.4 |
Isoelectric point (calc.) |
7.3 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LQRLVKALML/LEYDFRENLIL/LCKELLGPLRI |
Localization (YFP) |
nucleus>>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |