Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP4H10.13
Gene ID 39/G11
Gene synonyms/obsolete rps2302; rps23-2
Gene product 40S ribosomal protein S23
Entry clone Cloned
ORF length (unspliced) 432
ORF length (spliced)
Entry clone length 432
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP4H10.13.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGGTAAGCCCGCTGGATT
Rev primer name SPBP4H10.13.Rv
Rev primer SEQ AGAAAGCTGGGTAAGCTCTAGGTTTTTCCTTC
Amino acid length 143
Molecular weight 15.6
Isoelectric point (calc.) 11
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleolus>nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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