| Gene name |
SPBC2F12.11c |
| Gene ID |
40/A03 |
| Gene synonyms/obsolete |
rep2 |
| Gene product |
DSC1/MBF trancription
factor complex; zinc finger protein; zf-C2H2 type; involved in
start control point of mitotic cell cycle; interacts with the
MBC binding subunit complex; no apparent orthologs |
| Entry clone |
Cloned |
| ORF length (unspliced) |
660 |
| ORF length (spliced) |
|
| Entry clone length |
660 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC2F12.11.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCATTTTGCAGACATTCC |
| Rev primer name |
SPBC2F12.11.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAAAGAGCCAGTCATCTATC |
| Amino acid length |
219 |
| Molecular weight |
24.6 |
| Isoelectric point (calc.) |
9.3 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
155 |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
nucleus |
| Comments for localization |
|
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |