Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1A4.01
Gene ID 40/A07
Gene synonyms/obsolete apc10; SPBC1E8.06
Gene product anaphase-promoting complex (APC); regulators of the APC-cyclosome; involved in cyclin degradation (required); involved in metaphase-anaphase transition (required)
Entry clone Cloned
ORF length (unspliced) 699
ORF length (spliced) 570
Entry clone length 699
No. of intron 2
Sequence status Finished
Sequence results 301A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1A4.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCGCAAATTCGACAAGA
Rev primer name SPBC1A4.01.Rv
Rev primer SEQ AGAAAGCTGGGTACCGTAATTGGTTTCTACTT
Amino acid length 189
Molecular weight 21.4
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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