| Gene name |
SPBC20F10.06 |
| Gene ID |
40/A08 |
| Gene synonyms/obsolete |
mad2 |
| Gene product |
spindle assembly
checkpoint component; horma domain |
| Entry clone |
Cloned |
| ORF length (unspliced) |
699 |
| ORF length (spliced) |
612 |
| Entry clone length |
699 |
| No. of intron |
2 |
| Sequence status |
Finished |
| Sequence results |
42T:C / 477T:C /
604A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPBC20F10.06.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTAGCGTTCCCATAAG |
| Rev primer name |
SPBC20F10.06.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAGGATTCACTCGATATGCA |
| Amino acid length |
203 |
| Molecular weight |
23.5 |
| Isoelectric point (calc.) |
5.8 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
ER |
| Comments for localization |
accumulated ER by over
expression |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |