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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1815.02c
Gene ID 40/B04
Gene synonyms/obsolete cut2; SPBC14C8.01c
Gene product securin; involved in mitotic regulation; involved in spindle pole body checkpoint; involved in sister chromatid separation (required); essential
Entry clone Cloned
ORF length (unspliced) 906
ORF length (spliced)
Entry clone length 906
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1815.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCCTCCTCCTGTTCATTT
Rev primer name SPBC1815.02.Rv
Rev primer SEQ AGAAAGCTGGGTATAACAATCCTGTATCCAAA
Amino acid length 301
Molecular weight 32.8
Isoelectric point (calc.) 10.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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