Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC660.04c
Gene ID 40/C06
Gene synonyms/obsolete fbp1; SPBC1198.14c
Gene product fructose-1,6-bisphosphatase; glucose repression of transcription occurs by a cAMP signaling pathway; glucose repression of transcription is partially regulated by adenylate cyclase activation by a G protein alpha subunit encoded by gpa2; transcriptional regulators include two redundant Tup1p-like corepressors and the CCAAT binding factor activation complex
Entry clone Cloned
ORF length (unspliced) 1044
ORF length (spliced)
Entry clone length 1044
No. of intron 0
Sequence status Finished
Sequence results 677T:C / 723T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC660.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAAAAAAGATCTCGACGA
Rev primer name SPBC660.04.Rv
Rev primer SEQ AGAAAGCTGGGTATTTTATGAAATTAATATAT
Amino acid length 347
Molecular weight 38.5
Isoelectric point (calc.) 6.2
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol>nucleus
Comments for localization
Effect of LMB on protein localization changed to: cytosol=nucleus
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images
LMB 4 images) See all images

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