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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC337.08c
Gene ID 40/D01
Gene synonyms/obsolete ubi4
Gene product ubiquitin family protein; involved in meiosis (required)
Entry clone Cloned
ORF length (unspliced) 1149
ORF length (spliced)
Entry clone length 1149
No. of intron 0
Sequence status Finished
Sequence results Maybe another homolog
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC337.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCAGATTTTCGTCAAGAC
Rev primer name SPBC337.08.Rv
Rev primer SEQ AGAAAGCTGGGTAAAAAAGACCGCCACGAAGA
Amino acid length 382
Molecular weight 42.9
Isoelectric point (calc.) 7.8
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nuclear envelope; SPB; periphery; site of septum formation; nucleus>cytosol
Comments for localization observed by N-terminal tagging of GFP
Effect of LMB on protein localization not determined
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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