Gene name |
SPAC1A6.09c |
Gene ID |
40/D03 |
Gene synonyms/obsolete |
lag1 |
Gene product |
longevity-assurance
protein 1; lipid sensing domain; involved in intracellular
protein transport; involved in ceramide synthesis; similar to
Sp SPBC3E7.15C; involved in ER to golgi transport; involved in
GPI-anchored protein transport |
Entry clone |
Cloned |
ORF length (unspliced) |
1173 |
ORF length (spliced) |
|
Entry clone length |
1173 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC1A6.09.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGAGTAACCGAAAAGCTGA |
Rev primer name |
SPAC1A6.09.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATTTATCCTCATTCGTTGAA |
Amino acid length |
390 |
Molecular weight |
45.6 |
Isoelectric point (calc.) |
8.4 |
Signal SEQ |
|
No. of transmembrane domain |
7 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LCFWFLGLYI/LGFWLQQILVL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |