| Gene name |
SPCC11E10.01 |
| Gene ID |
40/D04 |
| Gene synonyms/obsolete |
SPCC61.06 |
| Gene product |
cystathionine
beta-lyase; involved in methionine biosynthesis from
L-homoserine via cystathione; cystathionine beta-lyase
activity |
| Entry clone |
Cloned |
| ORF length (unspliced) |
1173 |
| ORF length (spliced) |
|
| Entry clone length |
1173 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
1023T:G / 1052T:C /
1116T:C |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPCC11E10.01.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCCCAGCGATTGTAAATA |
| Rev primer name |
SPCC11E10.01.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAATTCTGTTTAAAATTTGCT |
| Amino acid length |
390 |
| Molecular weight |
42.7 |
| Isoelectric point (calc.) |
7 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no apparent
signal |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
DeltaVision |