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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC8E11.02c
Gene ID 40/D06
Gene synonyms/obsolete rad24
Gene product involved in DNA repair; involved in DNA damage checkpoint; involved in G2/M phase transition; 14-3-3 protein; negatively regulates Byr2p by affecting its localization; overexpression results in cell cycle defects; similar to Sp rad25
Entry clone Cloned in 2006 trial
ORF length (unspliced) 1220
ORF length (spliced) 813
Entry clone length 1220
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC8E11.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCTACTACTTCTCGTGA
Rev primer name SPAC8E11.02.Rv
Rev primer SEQ AGAAAGCTGGGTATGCGTCCGCCTTGGGCTCA
Amino acid length 270
Molecular weight 30
Isoelectric point (calc.) 4.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol; periphery at site of septum formation; SPB
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 3 images) See all images

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