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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC3H7.15
Gene ID 40/D07
Gene synonyms/obsolete hhp1
Gene product serine/threonine protein kinase; casein kinase I; involved in DNA repair (regulation) (required)
Entry clone Cloned
ORF length (unspliced) 1220
ORF length (spliced) 1098
Entry clone length 1220
No. of intron 1
Sequence status Finished
Sequence results 406T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC3H7.15.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCTTTGGACCTCCGGAT
Rev primer name SPBC3H7.15.Rv
Rev primer SEQ AGAAAGCTGGGTAATTAGGTCTGTTGATATAT
Amino acid length 365
Molecular weight 42.4
Isoelectric point (calc.) 9.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus; periphery at site of septum formation
Comments for localization cytoplasmic dots and nuclear dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 2 images) See all images

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