Gene name |
SPBC4B4.08 |
Gene ID |
40/F03 |
Gene synonyms/obsolete |
ght2 |
Gene product |
hexose transporter;
similar to Sp GHT1 and GHT3 and GHT4 and GHT5 and GHT6 and
SPCC548.06C and SPBC1348.14C |
Entry clone |
Cloned |
ORF length (unspliced) |
1596 |
ORF length (spliced) |
|
Entry clone length |
1596 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
129T:C / 508A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC4B4.08.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGGGTTCAAGCGTGGAAA |
Rev primer name |
SPBC4B4.08.Rv |
Rev primer SEQ |
AGAAAGCTGGGTATGCGTAATCTTCCTCATGG |
Amino acid length |
531 |
Molecular weight |
58.8 |
Isoelectric point (calc.) |
8.5 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
12 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
Golgi; periphery |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
DeltaVision |