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Schizosaccharomyces pombe Postgenome Database

Gene name SPBC1652.02
Gene ID 40/F11
Gene synonyms/obsolete aap1; SPBC16A3.20c
Gene product APC amino acid transporter
Entry clone Cloned
ORF length (unspliced) 1785
ORF length (spliced)
Entry clone length 1785
No. of intron 0
Sequence status Finished
Sequence results 392A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBC1652.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTTCGTTTACGGAATC
Rev primer name SPBC1652.02.Rv
Rev primer SEQ AGAAAGCTGGGTAGCAAAGGAAGTCGGCAACT
Amino acid length 594
Molecular weight 65.2
Isoelectric point (calc.) 8
Signal SEQ
No. of transmembrane domain 12
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LSAMILALIL
Localization (YFP) ER
Comments for localization accumulated ER by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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