| Gene name |
SPAC9E9.03 |
| Gene ID |
40/H05 |
| Gene synonyms/obsolete |
leu2 |
| Gene product |
3-isopropylmalate
dehydratase |
| Entry clone |
Cloned |
| ORF length (unspliced) |
2277 |
| ORF length (spliced) |
|
| Entry clone length |
2277 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
2199G:A |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC9E9.03.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTCCCTCAGTTGCTTC |
| Rev primer name |
SPAC9E9.03.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTACCAATCGTTCCTGCTAGAA |
| Amino acid length |
758 |
| Molecular weight |
82.7 |
| Isoelectric point (calc.) |
6 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
very dark signal of
cytosol |
| Comments for localization |
almost no apparent
signal |
| Effect of LMB on protein
localization |
no change |
| Microscope used for
observation |
DeltaVision |