Gene name |
SPBC17A3.04c |
Gene ID |
40/H09 |
Gene synonyms/obsolete |
pi043;
SPBC17A3.04c |
Gene product |
methionine-tRNA
ligase; involved in methionyl-tRNA aminoacylation;
methionine-tRNA ligase activity |
Entry clone |
Cloned |
ORF length (unspliced) |
2476 |
ORF length (spliced) |
2349 |
Entry clone length |
2476 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
100% match |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBC17A3.04.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGCAACCTATAAAGTTCA |
Rev primer name |
SPBC17A3.04.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGTTTGATCCATTACCACCA |
Amino acid length |
782 |
Molecular weight |
88.8 |
Isoelectric point (calc.) |
6.7 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
changed to:
cytosol=nucleus |
Microscope used for
observation |
DeltaVision |