| Gene name |
SPBC20F10.04c |
| Gene ID |
41/B09 |
| Gene synonyms/obsolete |
|
| Gene product |
conserved
hypothetical; hypothetical protein |
| Entry clone |
Cloned directly into
pDUAL-FFH1 |
| ORF length (unspliced) |
912 |
| ORF length (spliced) |
762 |
| Entry clone length |
912 |
| No. of intron |
4 |
| Sequence status |
Finished |
| Sequence results |
100% match |
| Comments |
|
| Polymerase used for cloning |
Pyrobest DNA Pol
(TaKaRa) |
| Fwd primer name |
SPBC20F10.04.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCCTCCATTGATAAACG |
| Rev primer name |
SPBC20F10.04.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTAAATTCTAAATATAATTGAT |
| Amino acid length |
253 |
| Molecular weight |
29.4 |
| Isoelectric point (calc.) |
10.9 |
| Signal SEQ |
|
| No. of transmembrane domain |
1 |
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
no expression
clone |
| Comments for localization |
|
| Effect of LMB on protein
localization |
not determined |
| Microscope used for
observation |
|