Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC31G5.03
Gene ID 41/C03
Gene synonyms/obsolete rps1101; rps11-1
Gene product 40S ribosomal protein S11
Entry clone Cloned
ORF length (unspliced) 459
ORF length (spliced)
Entry clone length 459
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC31G5.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGCCACAGAACTTGTCGT
Rev primer name SPAC31G5.03.Rv
Rev primer SEQ AGAAAGCTGGGTAGAATTTTCCAAATTGCTTG
Amino acid length 152
Molecular weight 17.5
Isoelectric point (calc.) 11
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol=nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 4 images) See all images

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