| Gene name |
SPAC589.08c |
| Gene ID |
41/C05 |
| Gene synonyms/obsolete |
|
| Gene product |
DASH complex;
microtubule-binding complex |
| Entry clone |
Cloned |
| ORF length (unspliced) |
468 |
| ORF length (spliced) |
|
| Entry clone length |
468 |
| No. of intron |
0 |
| Sequence status |
Finished |
| Sequence results |
154A:G |
| Comments |
|
| Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
| Fwd primer name |
SPAC589.08.Fd |
| Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGAGAAATACCAGAAAGC |
| Rev primer name |
SPAC589.08.Rv |
| Rev primer SEQ |
AGAAAGCTGGGTATCTGGAAGCGGAATAGGTT |
| Amino acid length |
155 |
| Molecular weight |
17.4 |
| Isoelectric point (calc.) |
4.5 |
| Signal SEQ |
|
| No. of transmembrane domain |
|
| NLS position (Columbia Univ.
Bioinformatics Center) |
none |
| NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
| Localization (YFP) |
SPB; dots on spindle
microtubules; nucleus>cytosol |
| Comments for localization |
|
| Effect of LMB on protein
localization |
changed to:
intranuclear microtubule bundle (cytosol=nucleus) |
| Microscope used for
observation |
DeltaVision |