Gene name |
SPAC4A8.15c |
Gene ID |
41/C06 |
Gene synonyms/obsolete |
cdc3 |
Gene product |
profilin; involved in
cytokinesis; involved in contractile ring assembly; involved
in actin filament polymerization; essential |
Entry clone |
Cloned |
ORF length (unspliced) |
489 |
ORF length (spliced) |
384 |
Entry clone length |
489 |
No. of intron |
2 |
Sequence status |
Finished |
Sequence results |
36A:G / 309G:A |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC4A8.15.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGTCTTGGCAAGGTTTGTA |
Rev primer name |
SPAC4A8.15.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATACCCAACACCAACAAGA |
Amino acid length |
127 |
Molecular weight |
13.4 |
Isoelectric point (calc.) |
5.8 |
Signal SEQ |
|
No. of transmembrane domain |
|
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
none |
Localization (YFP) |
nucleus>cytosol |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |