Gene name |
SPBPJ4664.05 |
Gene ID |
41/C09 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; conserved protein |
Entry clone |
Cloned |
ORF length (unspliced) |
492 |
ORF length (spliced) |
|
Entry clone length |
492 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
437T:C |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPBPJ4664.05.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGGTAGTAGGATTATTTTC |
Rev primer name |
SPBPJ4664.05.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAGGATCTTGGAGGTGCACCA |
Amino acid length |
163 |
Molecular weight |
18.4 |
Isoelectric point (calc.) |
8.7 |
Signal SEQ |
|
No. of transmembrane domain |
3 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LFSDISGSLTL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica,
DeltaVision |