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Schizosaccharomyces pombe Postgenome Database

Gene name SPBPB2B2.08
Gene ID 41/E01
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 663
ORF length (spliced)
Entry clone length 663
No. of intron 0
Sequence status Finished
Sequence results 324G:A
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBPB2B2.08.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATTGTACAACATAAAAC
Rev primer name SPBPB2B2.08.Rv
Rev primer SEQ AGAAAGCTGGGTATGAAAGACCTTTGAAAATG
Amino acid length 220
Molecular weight 25
Isoelectric point (calc.) 7.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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