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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPB24D3.04c
Gene ID 41/E04
Gene synonyms/obsolete mag1
Gene product DNA-3-methyladenine glycosylase; involved in nucleotide-excision repair; involved in DNA repair
Entry clone Cloned
ORF length (unspliced) 687
ORF length (spliced)
Entry clone length 687
No. of intron 0
Sequence status Finished
Sequence results 175G:A / 501A:T
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAPB24D3.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTTTGGACATTGAAGA
Rev primer name SPAPB24D3.04.Rv
Rev primer SEQ AGAAAGCTGGGTAGTGTTTCTTCGGCCTAACA
Amino acid length 228
Molecular weight 26.3
Isoelectric point (calc.) 9.4
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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