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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC20H4.11c
Gene ID 41/E07
Gene synonyms/obsolete rho5
Gene product Rho family; small GTPase; similar to Sp rho1
Entry clone Cloned#
ORF length (unspliced) 721
ORF length (spliced) 603
Entry clone length 721
No. of intron 1
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Pyrobest DNA Pol (TaKaRa)
Fwd primer name SPAC20H4.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGACTACTGAGCTCCGTCG
Rev primer name SPAC20H4.11.Rv
Rev primer SEQ AGAAAGCTGGGTAGAGTAAGATACAGTGCTTC
Amino acid length 200
Molecular weight 22.4
Isoelectric point (calc.) 6.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>=cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 1 images) See all images

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