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Schizosaccharomyces pombe Postgenome Database

Gene name SPCP20C8.01c
Gene ID 41/E10
Gene synonyms/obsolete
Gene product Sp specific families; B13958 domain; predicted coiled-coil; possibly Sp specific
Entry clone Cloned
ORF length (unspliced) 744
ORF length (spliced)
Entry clone length 744
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPCP20C8.01.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGATGTCGAACGAGAATTT
Rev primer name SPCP20C8.01.Rv
Rev primer SEQ AGAAAGCTGGGTATGCCAAATAACCTTGGTCT
Amino acid length 247
Molecular weight 29.6
Isoelectric point (calc.) 4.5
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) cytosol; cytoplasmic dots
Comments for localization bright dots by over expression
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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