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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC20H4.02
Gene ID 41/F01
Gene synonyms/obsolete
Gene product guanine nucleotide binding protein
Entry clone Cloned
ORF length (unspliced) 753
ORF length (spliced)
Entry clone length 753
No. of intron 0
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC20H4.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTCCTCAAGTGCTTTAAA
Rev primer name SPAC20H4.02.Rv
Rev primer SEQ AGAAAGCTGGGTACCATCGATACGAGTGTAAT
Amino acid length 250
Molecular weight 28.2
Isoelectric point (calc.) 4.5
Signal SEQ
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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