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Schizosaccharomyces pombe Postgenome Database

Gene name SPBP22H7.04
Gene ID 41/F04
Gene synonyms/obsolete pi027; SPACTOKYO_453.08
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 768
ORF length (spliced)
Entry clone length 768
No. of intron 0
Sequence status Finished
Sequence results 217A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPBP22H7.04.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGAGTATGCTAATCTTTAA
Rev primer name SPBP22H7.04.Rv
Rev primer SEQ AGAAAGCTGGGTAATATCGGTTTCCGATGACA
Amino acid length 255
Molecular weight 29.5
Isoelectric point (calc.) 10.6
Signal SEQ
No. of transmembrane domain 2
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LYFTLTLILFL
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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