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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC589.11
Gene ID 41/F09
Gene synonyms/obsolete
Gene product peptide chain release factor; tRNA hydrolase; involved in translation termination; similar to human ds-1 protein which shows modulated expression during colon carcinoma cell differentiation
Entry clone Cloned
ORF length (unspliced) 842
ORF length (spliced) 549
Entry clone length 842
No. of intron 1
Sequence status Finished
Sequence results 748T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC589.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTTTGCTAATTTCCGGAA
Rev primer name SPAC589.11.Rv
Rev primer SEQ AGAAAGCTGGGTAATCCATAGTGATACGACGT
Amino acid length 182
Molecular weight 21.4
Isoelectric point (calc.) 10.6
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) 161
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation DeltaVision

Image information
YFP 4 images) See all images

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