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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC20H4.05c
Gene ID 41/G10
Gene synonyms/obsolete
Gene product phosphate epimerase; similar to Sp SPAC9.06C
Entry clone Cloned
ORF length (unspliced) 923
ORF length (spliced) 666
Entry clone length 923
No. of intron 4
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC20H4.05.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGATGATTTTCTAAAGAA
Rev primer name SPAC20H4.05.Rv
Rev primer SEQ AGAAAGCTGGGTATTCTGGTTTTTGAAGCCTT
Amino acid length 221
Molecular weight 25.1
Isoelectric point (calc.) 6.7
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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