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Schizosaccharomyces pombe Postgenome Database

Gene name SPAC977.11
Gene ID 41/G12
Gene synonyms/obsolete
Gene product conserved hypothetical; hypothetical protein; telomeric duplication; similar to Sp SPBPB8B6.06; CRCB domain
Entry clone Cloned
ORF length (unspliced) 936
ORF length (spliced)
Entry clone length 936
No. of intron 0
Sequence status Finished
Sequence results 355T:C / 564T:C / 897A:G
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAC977.11.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGCTATTAACACAGTCTTA
Rev primer name SPAC977.11.Rv
Rev primer SEQ AGAAAGCTGGGTAATACTTTTCAGTATAACCA
Amino acid length 311
Molecular weight 34.5
Isoelectric point (calc.) 9.1
Signal SEQ Predicted (N-terminus)
No. of transmembrane domain 9
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) LGTLARLGL/LCANVFATLLL
Localization (YFP) ER
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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