Gene name |
SPAC977.11 |
Gene ID |
41/G12 |
Gene synonyms/obsolete |
|
Gene product |
conserved
hypothetical; hypothetical protein; telomeric duplication;
similar to Sp SPBPB8B6.06; CRCB domain |
Entry clone |
Cloned |
ORF length (unspliced) |
936 |
ORF length (spliced) |
|
Entry clone length |
936 |
No. of intron |
0 |
Sequence status |
Finished |
Sequence results |
355T:C / 564T:C /
897A:G |
Comments |
|
Polymerase used for cloning |
Platinum Taq HiFi
(Invitrogen) |
Fwd primer name |
SPAC977.11.Fd |
Fwd primer SEQ |
AAAAAGCAGGCTCTCATATGCTATTAACACAGTCTTA |
Rev primer name |
SPAC977.11.Rv |
Rev primer SEQ |
AGAAAGCTGGGTAATACTTTTCAGTATAACCA |
Amino acid length |
311 |
Molecular weight |
34.5 |
Isoelectric point (calc.) |
9.1 |
Signal SEQ |
Predicted
(N-terminus) |
No. of transmembrane domain |
9 |
NLS position (Columbia Univ.
Bioinformatics Center) |
none |
NES motif (
L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) |
LGTLARLGL/LCANVFATLLL |
Localization (YFP) |
ER |
Comments for localization |
|
Effect of LMB on protein
localization |
no change |
Microscope used for
observation |
Leica |