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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPB1A10.02
Gene ID 41/H08
Gene synonyms/obsolete
Gene product sequence orphan; hypothetical protein
Entry clone Cloned
ORF length (unspliced) 1011
ORF length (spliced)
Entry clone length 1011
No. of intron 0
Sequence status Finished
Sequence results 430A:G / 718T:C
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAPB1A10.02.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGTGCAATCGTCAACCGAA
Rev primer name SPAPB1A10.02.Rv
Rev primer SEQ AGAAAGCTGGGTAACATTTTTTTGACTCAGAA
Amino acid length 336
Molecular weight 37.6
Isoelectric point (calc.) 8.1
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) nucleus>cytosol
Comments for localization
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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