Chemical Genetics Laboratory
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Schizosaccharomyces pombe Postgenome Database

Gene name SPAPB1A10.03
Gene ID 41/H11
Gene synonyms/obsolete
Gene product involved in nuclear export; no apparent Sc ortholog
Entry clone Cloned
ORF length (unspliced) 1026
ORF length (spliced) 603
Entry clone length 1026
No. of intron 2
Sequence status Finished
Sequence results 100% match
Comments
Polymerase used for cloning Platinum Taq HiFi (Invitrogen)
Fwd primer name SPAPB1A10.03.Fd
Fwd primer SEQ AAAAAGCAGGCTCTCATATGGAGTCGAGTGTAAAATG
Rev primer name SPAPB1A10.03.Rv
Rev primer SEQ AGAAAGCTGGGTAATGGAATACATTCAAAAAA
Amino acid length 200
Molecular weight 23
Isoelectric point (calc.) 5.3
Signal SEQ
No. of transmembrane domain
NLS position (Columbia Univ. Bioinformatics Center) none
NES motif ( L-x(2,3)-[IVLMF]-x(2,3)-L-x-[LI]) none
Localization (YFP) mitochondrion?
Comments for localization too dark to photo
Effect of LMB on protein localization no change
Microscope used for observation Leica

Image information
YFP 2 images) See all images

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